"The ESCMID obserservship at the Charité - University Medicine Berlin was a great experience for me. I am greatly thankful to Dr. Trampuz and his group for their support and providing me an opportunity to gain new experience in the field of molecular diagnostics and infection control.
During my one month observership, I had an opportunity to observe and update my knowledge in the following area:
1. Research laboratory at the Charité - University Medicine Berlin and shadowed the new molecular techniques used for diagnosis of biofilms in implant-associated infection. These include bacteriophage experiment for eradication of biofilms, use of broad-spectrum and specific Multiplex PCR techniques for diagnosis of pathogens isolated from implants. Most importantly, I observed latest techniques such as sonication for isolation of biofilm-forming pathogens from implanted devices.
2. Labor Berlin - Molecular Diagnostic platform; I gained good knowledge about the workflow of diagnosis and all the molecular tests such as quantitative HIV, HCV RNA measurement of genotyping HCV and HPV and a multiplex PCR for the detection of respiratory pathogens, the molecular biological studies with accredited in-house methods that are performed. Moreover, I shadowed the laboratory design layout and biosafety system in the laboratory.
3. Infection control and prevention; I gained good knowledge about the strategies to prevent nosocomial spread of hospital-associated pathogens such as MRSA in the hospital. I discussed with Dr. Trampuz about the active and passive surveillance culture investigations, the study design and infection control definitions such as differentiating imported and acquired infections in the hospital. In addition to intervention programs such as promotion of hand hygiene, use of barrier precautions and microbiological procedures.
4. In the process of writing a review article about molecular diagnostics in implant-associated infections, as a product of my ESCMID observership visit.
Taken together, I gained good knowledge and experience in this supporting environment of Charité - University Medicine Berlin and encourages me to achieve my future career goals including establishing new molecular diagnostic technique laboratory and implementing a rigid infection control system in the hospital in my home country."
Soza Baban, Institute of infection, immunity & inflammation, Nottingham, United Kingdom